Question

Count data output by this technique is normalized as TPM or FPKM. For 10 points each:
[10m] Name this technique whose "single-cell" form yields data that can be processed via UMAP followed by clustering. This central technique of transcriptomics can identify differential expression.
ANSWER: RNA sequencing [accept single-cell RNA sequencing or scRNA-seq; prompt on sequencing; reject "DNA sequencing"]
[10e] RNA-seq is performed at this more coarse level of biological organization by the GTEx project. This broad category of structures, which compose organs, includes "muscle" and "epithelial."
ANSWER: tissues [accept tissue-specific]
[10h] Tissue-specific GTEx data can be used to call these genomic regions, which are distinct from GWAS hits. These regions are linearly correlated with continuous traits like gene expression.
ANSWER: quantitative trait loci [or QTLs or expression quantitative trait loci or eQTLs; or quantitative trait locus; prompt on quantitative characters]
<Science - Biology>

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